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These studies have also provided important insights into the molecular and cellular pathogenesis of NF and have served as a foothold for development of novel therapies for preventing or delaying tumor formation in NF patients [J Clin Invest, 131(1):e139807

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The cDNA was then combined with SYBR Green Master Mix and primers in appropriate proportions, amplified, and quantified using a real-time PCR system (BioRad, USA)

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