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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

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doi: 10.1016/j.ymthe.2016.11.020

That said, aside from drastic cases, where high stress causes a person to go grey fairly quickly, it is often difficult to say whether grey hairs are appearing because of age, stress, or a combination of both