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Detailed descriptions of the preparation of the cDNA inserts and creation of the gene fusion is beyond the scope of this discussion, but rely on standard molecular biology techniques such as those described in (11, 12)

(PubMed) Shults CW, Oakes D, Kieburtz K, et al

Seven of the 19 XChem hits were shown to bind to the protein, but with only a modest average peak intensity reduction of 2037% in the CPMG assay (Supplementary Table 2), suggesting that the fragments likely bound with weak (millimolar) affinities, at the very limit of the CPMG assay sensitivity

However, it is not only possible to combine SA with other chemicals

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10.1016/j.pharmthera.2020.107476 130