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GLYI is the most important enzyme for MG detoxification BY4741, GLYIKO, GLYIIKO and D-LDHKO cells containing empty vector were grown on Ura SD agar plates with either glucose or galactose and different concentrations of MG

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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12

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