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The content below explains the scientific background, proposed research mechanisms, typical application areas, storage expectations, reconstitution considerations where applicable, and quality-control points that support careful experimental planning

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Substances That Aggravate RLS Several common substances increase nervous system excitability: Caffeine stimulates dopamine release earlier in the day and worsens nighttime rebound symptoms Alcohol disrupts sleep architecture and increases nighttime awakenings Nicotine increases sensory sensitivity and delays nervous system relaxation Certain medications, including some antidepressants and antihistamines, may also exacerbate RLS and should be reviewed with a healthcare provider

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