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Mammalian cell-based assay to identify coronavirus protease inhibitor (SARS-CoV, SARS-CoV-2, and MERS) Expression constructs of SARS-CoV, SARS-CoV-2, or MERS 3CL protease (Twist Biosciences) were cloned into pLEX307 backbone (Addgene #41392) using Gateway LR II Clonase Enzyme mix (Invitrogen, 11791020) and transformed into NEB 10-beta high-efficiency competent cells

Villacres MC, Longmate J, Auge C, Diamond DJ

Revel-Vilk S

The 5 g vial of hydroxocobalamin for injection is to be reconstituted with 200 mL of 0.9% NaCl, to give a dark red injectable solution (25 mg/mL)

doi: 10.1097/md.0000000000037114 170 ZhengPZengBLiuMChenJPanJHanYet al

4,5 A key advantage in the study of APAP toxicity is that its human pathophysiology can be effectively reproduced in a mouse model in vivo and in primary mouse hepatocytes