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explored the use of different substituted thienyl and furyl moieties other than the 5-bromo-2-thienyl group at C5 of the THC imidazolidinedione scaffold while keeping the other structural features of compound 17 (an S -configuration at C5 and an ethyl substituent at the terminal nitrogen), the 5-ethyl-2-furyl group in compound 18 showed the highest activity with equal potency to tadalafil (IC 50 of 2.92 nM)
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Lentiviral-mediated shRNA interference All shRNAs for GCLC and SIRT2 were either from the reported literature [62] or designed and purchased from Sangon Biotech
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