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The LET7 PCR products were digested by BamHI and StuI and ligated into the plant protoplast expression vector pHBT under a CaMV 35S ( p35S ) promoter with an HA, FLAG or GFP tag at the C terminus to obtain pHBT -p35S :: LET7-HA , p35S :: LET7-FLAG or pHBT -p35S :: LET7-GFP

Besonders in Zeiten erhhter Belastung kann dies von unschtzbarem Wert sein

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