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In addition, fumarate inhibits TET enzymes, which stimulate EMT, leading to cancer metastasis 131,132

first proposed the concept of oxidative stress, either caused by an increase in free radical production or a reduction in the scavenging capacity of the body, leading to disorders in the oxidation and antioxidant systems, resulting in oxidative damage by free radical accumulation (Sohal and Allen, 1990)

Brazil Hydrol Process 17:14431450

Marzani et al

Protein extraction and western blot analysis Cultured cells were lysed in radioimmunoprecipitation assay lysis buffer (Beyotime, Shanghai, China) supplemented with phenylmethylsulphonyl fluoride and protease inhibitor cocktail (Sigma-Aldrich, USA) on ice for 10 min and centrifuged at 12,000 g for 10 min at 4 C

Independent Verification: Every batch is tested to confirm the absence of heavy metals and cross-contaminants