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It effectively inhibits tyrosinase activity, the enzyme responsible for melanin production, leading to a more even skin tone and reduced hyperpigmentation

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10.3390/nu14173566 Nutrients 83 LiuS.LiE.SunZ.FuD.DuanG.JiangM.et al (2019)

Cells were lysed with NIB-250 buffer (15 mM Tris-HCl (pH 7.5), 60 mM KCl, 15 mM NaCl, 5 mM MgCl 2 , 1 mM CaCl 2 , 250 mM sucrose, 1 mM DTT, 10 mM sodium butyrate, protease inhibitors) with 0.1% NP-40 for 5 minutes on ice
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