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Levels of HMGB1::Lucia in the supernatant can be readily monitored by measuring the light signal produced after the addition of QUANTI-Luc 4 Lucia/Gaussia, a Lucia luciferase detection reagent ( see figure )
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While monotherapy may not display an optimistic clinical benefit or safety profile, harnessing combination approaches to maximize the antitumor effects while minimizing toxicities seems to be a promising strategy for cancer treatment
