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After addition of 250 l of RPMI-1640 medium, 300 U/ml penicillin, and 175 g/ml streptomycin, parasites were incubated at 37 C in 5% CO 2 atmosphere for 4 d

Beyond direct H 2 O 2 scavenging, GSH also detoxifies lipid peroxidation byproducts, including malondialdehyde (MDA) and 4-HNE, through GST-mediated conjugation, facilitating their removal and preventing secondary cytotoxic signaling 91,92

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