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pTRIP-CMV-EB3-mScarlet-I was constructed via Gibson assembly of EB3-mScarlet into the pTRIP-CMV-GFP-2A vector opened at the NheI and KpnI sites

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The compound incorporates four strategic amino acid substitutions compared to the native GHRH sequence, improving its resistance to enzymatic breakdown while preserving potent receptor binding

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