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This framework supports reliable physicochemical performance, including solubility behavior and stability in experimental conditions, enabling consistent results in signaling, metabolic, and biochemical assays

While cytosol, peroxisomes, mitochondria, and plastids in Arabidopsis wild-type plants maintain a highly reduced glutathione buffer with redox potentials of less than 310 mV, the ER is far less reducing with E GSH values of less than 240 mV (Meyer et al., E GSH with high specificity due to selective mediation of thiol/disulfide exchange reaction between glutathione and roGFP by GRXs (Meyer et al., E GSH is outside the measuring range of these probes thus limiting their usability

Szargel R, Shani V, Elghani FA, Mekies LN, Liani E, Rott R, et al

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