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Cell viability was evaluated by MTT assay, apoptosis by flow cytometry using annexin-V/propidium iodide staining, and protein expression of apoptosis-related markers (Bax, Bcl-2, and caspase-8) by western blot

4-Hydroxynonenal (4-HNE) is an , unsaturated hydroxyalkenal and an oxidative/nitrosative stress biomarker

Vitamin B12 deficiency is a widespread condition, particularly among elderly individuals, patients with malabsorption syndromes, and those following plant-based diets