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The membrane was then blocked in 5% non-fat milk in 1x TBS-T (Cat# 03-500-537) for 60 minutes

522 One of the most critical aspects of these long-term adaptations is the alteration in neurocognitive function, which manifests as slowed reaction times and decreased processing speed

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Supernatants were subjected to Western blot analysis, to verify successful Coeae6g expression (Supplementary methods