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The mass spectrometer method was operated in positive-ion mode with a 4-s cycle time data-dependent acquisition with advanced peak determination and Easy-IC on (internal calibrant)

doi: 10.1016/0014-2999(91)90762-F

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These products would potentially enter the TCA cycle for further metabolism, which was confirmed by the up-regulation of related enzymes

Step 6: Withdraw the needle smoothly

When your levels drop, everything slows down