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Saline (0.9% NaCl): While sterile, the salt content can sometimes cause certain complex peptides to clump or degrade faster

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AlphaFold predictions The protein sequences of JFH1 NS3, NS3h and NS5B and RNA sequences from the JFH1 3X (the last 98 nucleotides in the 3UTR) or from 5BSL3 (nucleotides 9281-9423) were used as input for AlphaFold3 (AF3) 67 through the beta AF3 server at Results were downloaded locally and visualized with Pymol (The PyMOL Molecular Graphics System, Version 3.0, Schrdinger, LLC)