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Membranes were then probed with horseradish peroxidase-conjugated secondary antibodies antirabbit IgG (Abcam, A16035, 1:5,000) or antimouse IgG (Abcam, A16011, 1:5,000) and developed using enhanced chemiluminescence substrate (Bio-Rad, 170-5060)

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Studies in knock-out mice, however, suggest that the homologous MRP4 (ABCC4) is more likely to be the physiological efflux transporter of this eicosanoid (76)