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While all GSTZ1-1 variants are inactivated by DCA, one variant, which has lysine instead of glutamic acid at position 32 and arginine instead of glycine at position 42 of the protein, is more rapidly inactivated than other variants in the presence of physiological concentrations of chloride

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The CaMV 35S minimal promoter (-46 to +7) fused to the GUS gene from pBT-GUS [45] was cloned as a BamHI and XmaI fragment into pPAM (GenBank AY027531)

Mice were divided into groups ( n = 6 per group) receiving AAV9-empty vector (AAV9-Ctrl), AAV9- Ksp - Usp2 ( Usp2 OE group), AAV9- Ksp - Usp2 K447R (mutant group), and AAV9- Ksp -short hairpin RNA targeting Usp2 (AAV-shUsp2)

Vah Haren RM, Sorrell LP, Sepesi B, Rice D, Hofstetter W, Roth J, Swisher SG , Walsh G, Vaporciyan AA, Mehran RJ, Antonoff MB
