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A High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) was used to perform the reverse transcription following the manufacturers instructions and using the ProFlex PCR System machine (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA)

Vitamin B12 is primarily sourced from meat, making deficiencies more common among: Vegans and vegetarians Busy and active mums Individuals recovering from surgery Those on weight loss journeys Regular injections throughout the year or a structured course of treatment can help restore and maintain your vitamin levels

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