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This method is a mild elution using a buffer with increased ionic strength usually sodium chloride, applied as a linear gradient or in steps

This results in cellular inflammatory injury, apoptosis, and fibrosis

The DBA/2J mouse strain, which develops a similar phenotype of chronic glaucoma, has mutations in the Gpnmb gene (encoding a lysosomal-related glycosylated protein) and the Tyrp1 gene (encoding a melanosomal protein with possible enzymatic and structural functions) (38, 39)

Kadia T, Estrov Z, Ravandi F, Koller C, Borthakur G , Jabbour E, Faderl S, Pierce S, Cortes J, Kantarjian H, Garcia-Manero G

Zimmerman, M., Gottster, A

Canonically, as an exogenous ligand (e.g