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De Siqueira MK, Li G, Zhao Y, Wang S, Ahn IS, Tamboline M, et al
ALA preserve the enzyme activities of pyruvate dehydrogenase, and -ketoglutarate dehydrogenase through binding with amide linkage at lysine amino acid sites
When working with a smaller protein, roughly 20 kDa and less, you can likely add any of these tags and get soluble expression in E
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