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When Trx is in a reduced state, Trx-(SH) 2 , the two active-site cysteines form a dithiol group that is able to catalyze the reduction of disulfides in a number of proteins

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Heales SJR, Davies SEC, Bates TE, et al (1995) Depletion of brain glutathione is accompanied by impaired mitochondrial function and decreased N -acetyl aspartate concentration

The absorbance of the cells in the wells was then measured at 490 nm using the Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, Inc.) to evaluate the cell viability