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doi: 10.1186/s12263-018-0605-7 88

These results prove the potential to use this methodology beyond the nanomolar range, as showcased before, for an upscale at high concentrations, reduced amount of reagent and with an inorganic base

[Google Scholar] 40.Mckeague J.A., Wolynetz M.S

For this purpose, cells grown on glass coverslips were loaded with fura-2 by a 30 min incubation at 37 C with 1 M fura-2-AM and 0.02% Pluronic in the extracellular solution described above
It may be especially noted that, in the active site of enzymes, the contribution of solvation from water is usually minimal and thus the information gained by exploiting some sophisticated techniques of modern mass spectrometry may better contribute to understanding biochemical mechanisms

[DOI] [PMC free article] [PubMed] [Google Scholar] 174.Wang S.M., Han C., Lee S.J., Patkar A.A., Masand P.S., Pae C.U
