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Despite elevated intracellular GSH levels and increased redox cycling activity, the transcriptomic data indicated downregulation of Gss (glutathione synthetase), Gclc (glutamate cysteine ligase catalytic), and Chac1 (a canonical GSH-degrading enzyme), alongside significant upregulation of Chac2, a less characterized homolog involved in glutathione regulation (Fig

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These enzymes insert Co 2+ into sirohydrochlorin to generate Co(II)-sirohydrochlorin but are structurally related to the iron-inserting enzyme SirB that converts sirohydrochlorin into siroheme [157,163,164], as well as to CbfA, which is the nickel chelatase that inserts Ni(II) into sirohydrochlorin for coenzyme F 430 biosynthesis [165,166]