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In the sample with autoimmune hypothyroidism, the diagnosis had been made by endocrinologists at the Helsinki University Central Hospital, where the diagnostic criteria included elevated TPO antibodies, elevated TSH, reduced FT4, and ultrasound findings characteristic of autoimmune thyroiditis

The membranes were washed and the ELISA substrate added (Boehringer Mannheim, Laval, QC, Canada)

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n = 459, RCT = 7, P 0.05), while Sigman et al