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This is driven by the likelihood of subchondral bone changes as a consequence of failed repair, particularly if the first attempt involved disruption of the tidemark for marrow recruitment

PLoS ONE 8:e83466

The SpO2/FiO2 ratio has been clinically validated for diagnosing and stratifying ARDS patients, but it may be less accurate in poor perfusion states, above 97% oxygen saturation, and in patients with darker skin pigmentation

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84 Importantly, next-generation sequencing and methylation assays have identified key genetic alterations in ctDNA, including mutations in genes such as p53 , EGFR , MET , PIK3CA , and NOTCH1 , highlighting the feasibility of liquid biopsies in molecular profiling and personalized therapy selection

Normalization and relative quantification were conducted using the 2 Ct method (Livak and Schmittgen, 2001)