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lactis biovar)

For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, H-3300-250)

However, that's not it

Preliminary work carried out by Nossal and Lederberg indicated that one cell makes only one antibody
Hertfordshire, United Kingdom

After tipping off the supernatant, diluted serum samples were incubated for 2 h at RT
