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Ahn SH, Khalaj K, Young SL, Lessey BA, Koti M, Tayade C
Eluted protein was further purified by HiTrap Heparin HP affinity column (Cytiva, 17040703) with a linear gradient of Low-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 50 mM NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) and High-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 1 M NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) to remove RNA contamination

Srivastava et al., 2020b)

April 2024

Similarly, genealogic analyses and twin studies have shown a genetic link between ME/CFS patients and their offspring, ensuing a heightened risk of developing ME/CFS in the offspring (26, 66, 69)

14, 17371751, 10.1093/cvr/cvae128