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Advanced biochemical and histochemical detection techniques such as DCFDA-based fluorometric assays, NBT and DAB staining, and TBARS quantification have become indispensable tools for investigating ROS dynamics under various abiotic and biotic stress conditions
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The important one is that affinity chromatography involves many types of interactions between ligand and target such as steric effects, hydrogen bonding, ionic interactions, van der Waals forces, dipol-dipol interactions and even covalent bonds while other chromatographic techniques involve just one or a few of them
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