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400 L of the supernatant was added to ice-cold Folch solution (2:1 v/v chloroform: methanol) for lipid extraction, and 100 L was used to determine protein concentration using BCA Protein Assay Kit (Thermo Fisher)

Although the BCFA content in the human diet may never reach the total lipid percentages used in our experiments, BCFAs can also be synthesized from branched chain amino acids (BCAAs) by human cells and be produced by the gut microbiota

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Chronic Oral L-Carnitine Supplementation Drives Marked Plasma Tmao Elevations in Patients with Organic Acidemias Despite Dietary Meat Restrictions