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After culturing for 30 h, cells were stained with 5 g ml 1 calcein-AM at 37 C for 15 min

As there are no known disadvantages associated with the oral intake of the above-mentioned antioxidant vitamins, the main advantage lays in the fact that they present good safety profiles and are free from harmful side effects (Diplock, Reduction of the Cochlear Blood Flow and Vasodilators Exposure to loud noise, in addition to inducing oxidative stress, also diminishes cochlear blood flow by reducing blood vessel diameter and erythrocyte velocity in the basilar membrane, the spiral ligament and the SV (Sendowski, 2006

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Its also something we tend to produce less of as we get older