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Labonia, W
SinhaTVich VilaAGarmaevaSJankipersadsingSAImhannFCollijVet al
[DOI] [PMC free article] [PubMed] [Google Scholar] 36.Yui K., Sato A., Imataka G
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BMC Med Res Methodol
Validation of Primers Quantitative RT-PCR assays were initially optimized to give PCR efficiency between 1.9-2.1 (as determined by a 3-point standard curving using serial dilutions of control RNA with a slope range of 2.9 to 3.5) and a single melt-peak corresponding to the appropriate PCR product as verified by 2 % agarose gel electrophoresis
