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After PBS washing, sections were incubated with an HRP-conjugated, species-matched secondary antibody (50 min, room temperature), followed by further PBS washes
As a water-soluble vitamin, excess amounts are excreted through urine
PMID: 167 HiguchiBSRodriguesNGonzagaMICicogna PaioloJCStefanuttoNOmoriWPet al
Cells treated with the indicated siRNAs were stained with the cell-permeable DNA stain SiR-Hoechst and the membrane-impermeable DNA dye SYTOX Green which can only enter cells with compromised cell membrane integrity
Petra Hinterleitner and George Mare for assisting sample preparation
10.1016/s0928-4257(01)00044-4 57 RobertA.NezamisJ
