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The dysregulation of calcium homeostasis/signaling and apoptosis is thus likely the cause of the pupae lethality observed in Regucalcin RNAi experiments (see above)

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Cells were harvested and analyzed by the flow cytometry or microplate reader (Ex/Em = 488 nm/525 nm)

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Intracellular localization (plasma membrane versus intracellular organelles) might thus depend on the level of protein expression resulting in more pronounced accumulation at the plasma membrane when expression is stimulated

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