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Its two-step synthesis glutamate-cysteine ligase (GCL, rate-limiting, producing -glutamylcysteine at the cost of one ATP) followed by glutathione synthetase (adding glycine, consuming a second ATP) produces a tripeptide whose unusual -glutamyl linkage between glutamate's -carboxylate and cysteine's amine renders it resistant to cellular peptidases, enabling accumulation to 0.515 mM concentrations in most cell types

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Materials and methods Instrumentation and characterization

10.1007/s13311-021-01170-y [DOI] [PMC free article] [PubMed] [Google Scholar] Ryan F., Blex C., Ngo T

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It is important to consider that prolonged high cholesterol diet (HCD) can lead to chronic systemic inflammation, resulting in an increased accumulation of dysfunctional and senescent immune cells (89)