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The qPCR cycling conditions were 95 C for 5 min followed by 38 cycles of 95 C for 10 s and 60 or 63 C for 30 s

[DOI] [PMC free article] [PubMed] [Google Scholar] Demaria M, Ohtani N, Youssef SA, Rodier F, Toussaint W, Mitchell JR, Laberge RM, Vijg J, Van Steeg H, Dolle ME, et al

Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific)

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Booster injections are administered by trained medical professionals using sterile techniques
Additionally, DJ-1 -deficient embryonic cortical neurons show increased sensitivity to oxidative damage
