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Following centrifugation (13,000 rpm, 15 min, 4 C), supernatants were mixed with 10 mM K-phosphate buffer pH 7 and 1 M potassium iodide (KI), then incubated in the dark for 15 min
10.1158/0008-5472.can-08-3530 168 SinclairP
Consequently, the emerging homozygous ura3 mutants could dominate the population within 50 generations of culture with 5-FOA
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A., & Belsito, D
