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However, due to the small sample size and the suboptimal experimental study design with significant methodological limitations, these results require caution and need to be replicated in larger well-performed clinical trials

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14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

Previous research has shown that GPX4 plays a reno-protective role in tubular cells, with GPX4-null mice exhibiting marked increases in tubular cell death and proteinuria (12)