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Late assessment: rats with ligated left colic artery and vein that underwent additional colon obstruction for three days (IC + OB rats) In deeply anesthetized rats, in addition to the left colic artery and vein obstruction by two ligations performed as described above, which resulted in a 25-mm blood-deprived colon segment, an additional colon obstruction was made close to the distal ligation using a plastic ring 4 mm in diameter

IV infusion (clinic-based) Protocol: Continuous daily supplementation -- typically 250-1000 mg NMN or 300-600 mg NR per day Rationale: NAD+ depletion is continuous, requiring ongoing replenishment to maintain elevated levels Requirements: No special handling for oral supplements

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During this period, no adverse effects emerged with continued use

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