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The E8 medium was replaced every day and cells were passaged twice a week using TrypLE Select

The downregulation of KLF2 expression is significantly correlated with the TNM staging of ccRCC, and ccRCC patients with low expression of KLF2 have significantly shorter overall survival and metastasis-free survival periods

Paracellular permeability is regulated by the tight junction proteins, which determine the size and charge of molecules that are able to pass between the epithelial cells [22]

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doi: 10.1681/ASN.2021060843 188