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G.Correa-BasurtoJ.Rosales-HernandezM

After washing with 1 ml PBS solution, cells were further crosslinked with 3 mM disuccinimidyl glutarate (Fisher Scientific) in 2.5 ml PBS solution at 30 C for 40 min

Furthermore, lipid nanoparticles (LNPs) efficiently encapsulate and protect mRNA, facilitating its transfection and intracellular delivery, and thereby offering future opportunities to deliver exercise-induced factors or gene-encoded mimetics across the BBB [38]

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