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Following immunostaining, L3 central nervous system (CNS) or body walls were mounted in SlowFade Gold (Invitorgen) and fluorescent images were acquired on a LSM 980 (Carl Zeiss) using a 20 objective (for the CNS) or 40/63 objective (for the NMJ)

Your endocrine system responds to these signals the same way it did when you were younger, before age-related decline began eroding your baseline GH production by roughly 15% each decade after your thirties

Immunity 55 , 22362254 (2022)

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