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Primary hepatocytes were infected with Ad-TetOn-NHK or Ad-CMV-eGFP at a multiplicity of infection of 1:1

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These heightened responses are mediated not only by increased glutamatergic (as opposed to GABAergic) activity but also increased anxiogenic peptidergic activity [i.e., corticotrophin releasing factor (CRF) as opposed to anxiolytic neuropeptide Y (NPY) activity] within the extended amygdala, particularly the AMYG and BNST (Roberto et al., 2012)