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Additionally, since the longer chain humectants will sit on the top of the skin and bind reversibly to smaller humectants (via whats called hydrogen bonding), the smaller humectants will gradually absorb into the skin over a longer period of time, like an extended release humectant system, so the plumping and hydration effects last longer

After centrifugation, the supernatant was collected in a new tube

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10.1016/j.bbagen.2012.11.008 57 KiernanA

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Additionally, mobility changes were observed after neuraminidase digestion, consistent with the loss of 8 terminal sialic acids associated with the N-glycans (Fig