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Mol Gen Genomics 272:639650 Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-DeltaC(T)) method
[8]Gong D, Bi Y, et al
As there was no differences of islet area nor islet density between groups (Fig
The pellet (precipitated proteins) was solubilized in 300 l of 100 mM phosphate buffer containing 2 mM -mercaptoethanol and 10% glycerol, and stored at 80C
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