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Absorbance at 610 nm was recorded on a plate reader (Synergy, BioTek)

Gomperts, R

Resources, P.W.R.H., J.S., J.W

The rationale for titrating upward over time, rather than starting at the target dose, mirrors the approach taken with other metabolic peptides: the goal is to identify the minimum effective dose for each individual while monitoring for tolerability

Wait for one minute then massage the area of injection gently for FIVE MINUTES to disperse the B12
