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Comparison of old /new TNR epitope localization with 2-color STED To create the images of the average dendritic spine, image segments containing individual synaptic puncta were manually aligned based on the DiO channel (see Quantification of TNR at synapses by STED and confocal microscopy)

Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria

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doi: 10.3390/ijms23095144

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Following 30 min of incubation at 4 C, extracts were cleared by centrifugation (17,000 g , 20 min, 4 C) and then supernatants were analysed by liquid chromatography and mass spectrometry (LCMS)