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Male SAMP8 mice develop LV hypertrophy with diastolic dysfunction as early as 6 months of age ( 2.2.3 Fischer 344 rats (H 2 FPEF score 3) The aged Fischer 344 rats develop pathophysiology of aging in various organs, including the heart (Walker et al., 2006) with evident pulmonary congestion and exercise intolerance at 25 months of age (Suen et al., 2019) by 28 months of age, these animals also develop LV systolic dysfunction and chamber dilation, which is infrequently seen in human HFpEF (Walker et al., 2006), thus limiting the use of Fischer 344 rats as an HFpEF model